Protein Extraction
Phenomenex Inc Phenomenex Lysis Loading Buffer, Clarity OTX Pro, 100 mL
Clarity OTX Pro Lysis Loading Buffer is a ready-to-use lysis and loading reagent formulated to prepare biological samples for Clarity OTX Pro solid-phase extraction. It lyses cells, denatures proteins, inhibits nucleases and proteases, and conditions samples for reliable SPE extraction and LC/MS analysis of therapeutic oligonucleotides and their metabolites.
- Provides cell lysis, liposome disruption, and protein denaturing.
- Inhibits nucleases and removes protease activity in biological fluids.
- Formulated for extraction of therapeutic oligonucleotides and metabolites from plasma, serum, urine, sputum, and tissue.
- Ready-to-use, 100 mL volume, pack size: each.
- Recommended sample ratios: at least 1:1 buffer to biological fluids; approximately 3-5x buffer to tissue; target pH ~5.5 after mixing.
- Contains Triton X-100 and guanidine hydrochloride; handle according to safety data sheet.
Qiagen, Inc. BUFFER G2 250ML
Buffer G2 (260/250),KG (Encompass)
Sigma Aldrich Fine Chemicals Biosciences RIPA Buffer
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
Enzo Life Sciences RIPA cell lysis buffer 2, (100ml)
Formulation: Liquid. 50mM TRIS-hydrogen chloride, pH 7.4, containing 150mM sodium chloride, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 1% sodium deoxycholate and 0.1% SDS. Long Term Storage: +4°C.
Sigma Aldrich Fine Chemicals Biosciences CelLyticTM Y Cell Lysis Re
CelLytic Y Cell Lysis Reagent is versatile phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.
Raybiotech Inc Lysis Buffer, 16 mL, 2X
16 mL of 2X Lysis Buffer. 2X Lysis Buffer should be diluted 2-fold with deionized or distilled water. Supplementation with protease or phosphatase inhibitors is recommended. You may use RayBiotech Protease Inhibitor Cocktail (AA-PI), RayBiotech Phosphatase Inhibitor Cocktail Set I (AA-PHI-I) or Phosphatase Inhibitor Cocktail Set II (AA-PHI-II), or the RayBiotech Phosphatase / Protease Inhibitor Set (AA-PIPHI). Alternatively, you can purchase inhibitor cocktails from another manufacturer and follow their specifications.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES CELLYTIC MEM PROTEIN EXTRACTIO
NC3523468 CELLYTIC MEM PROTEIN EXTRACTIO
Bioworld Yeast Lysis Solution, 500 mL
Yeast Lysis Solution-DNA Isolation Common solution for protein and DNA isolation from yeast. Technical Information:LiCl (2.5M)Tris-HCl, pH 8.0 (50mM)Triton X 100 (1%)EDTA (62.5mM)
TARGETMOL CHEMICALS INC RIPA-56 50MG
Also available in 5 mg 10 mg 25 mg 100 mg 200 mg 500 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. RIPA-56 is an inhibitor of RIP1purity: 98%
Biotium 5X Passive Lysis Buffer
5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium's Renilla Luciferase Assay Kit 2.0. Those kits are supplied with sufficient 5X Passive Lysis Buffer to complete the stated number of assays using 96-well to 24-well plates. 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g. >100 uL per well) is required.